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Updated: May 4, 2026

Protocol for Biofilm Streamer Formation in a Microfluidic Device with Micro-pillars
Published on: August 20, 2014
Biofilm formation in water cooling systems
M T Lutterbach1, F P de França
1, .
Abstract:
Biofilm formation on stainless steel samples immersed in cooling water has been evaluated by exposing metal samples to cooling seawater for 30 days. Anaerobic bacteria were then at 1.6 × 10(6)/cm(2), with sulphate-reducing species predominating. Aerobic bacteria and fungi were 2600 and 140/cm(2), respectively. After 60 days, numbers of aerobic microorganisms remained constant whereas the count of anaerobic microorganisms had increased to 1.8×10(9)/cm(2). Scanning electron microscopy showed the presence of morphologically different microorganisms in deposits and as a mucilaginous net. No signs of corrosion were detected on the stainless steel surface.
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