Related Experiment Video

Updated: May 4, 2026

Customization of Aspergillus niger Morphology Through Addition of Talc Micro Particles
10:51

Customization of Aspergillus niger Morphology Through Addition of Talc Micro Particles

Published on: March 15, 2012

21.9K

Short communication: Influence of inoculum preparation on citric acid production by Aspergillus niger

M G Fernández Vergano1, M A Soria, N L Kerber

  • 1, .

World Journal of Microbiology & Biotechnology
|January 14, 2014
PubMed

Insights

Optimizing Aspergillus niger spore cultivation is key for citric acid production. Shu & Johnson agar (SJA) and shorter incubation times (under 7 days) enhance spore viability and yield.

Area of Science:

  • Microbiology
  • Biotechnology
  • Industrial Fermentation

Background:

  • Citric acid is a vital industrial chemical produced by Aspergillus niger.
  • Optimizing spore viability and citric acid yield requires careful control of cultivation parameters.

Purpose of the Study:

  • To investigate the impact of sporulation medium and incubation time on Aspergillus niger spore viability and citric acid production.
  • To identify optimal conditions for enhanced citric acid biosynthesis.

Main Methods:

  • Cultivation of Aspergillus niger spores on different media: Shu & Johnson agar (SJA), potato dextrose agar, and malt-extract agar.
  • Incubation of spores for varying durations.
  • Measurement of spore viability and citric acid titres.

Main Results:

  • Shu & Johnson agar (SJA) and potato dextrose agar supported higher citric acid titres compared to malt-extract agar.
  • SJA demonstrated superior spore germinability.
  • Spore viability increased with extended incubation, but optimal citric acid production occurred with incubation periods under 7 days.

Conclusions:

  • The choice of sporulation medium significantly influences citric acid production and spore germinability in Aspergillus niger.
  • Shorter incubation times (less than 7 days) are favorable for maximizing citric acid yield, despite increasing viability with longer incubation.

More Related Videos

Quantitative Analysis of Aspergillus nidulans Growth Rate using Live Microscopy and Open-Source Software11:30

Quantitative Analysis of Aspergillus nidulans Growth Rate using Live Microscopy and Open-Source Software

Published on: July 24, 2021

3.3K
Author Spotlight: Enhancing AM Fungi Research with SAP-AS — A Novel Technique for Single-Spore Cultures06:43

Author Spotlight: Enhancing AM Fungi Research with SAP-AS — A Novel Technique for Single-Spore Cultures

Published on: June 14, 2024

2.6K

Related Concept Videos

The Citric Acid Cycle: Overview01:37

The Citric Acid Cycle: Overview

In aerobic organisms, the citric acid cycle is the second stage of cellular respiration wherein molecules derived from the breakdown of carbohydrates, proteins, and fats are oxidized into carbon dioxide and energy. This process is also known as the tricarboxylic acid (TCA) cycle as the first product of the cycle, citric acid, contains three carboxyl groups in its structure. Alternatively, this cycle is also referred to as the Krebs cycle, in honor of its discoverer Sir Hans Krebs.
The citric...
14.7K
Products of the Citric Acid Cycle00:53

Products of the Citric Acid Cycle

The cells of most organisms—including plants and animals—obtain usable energy through aerobic respiration, the oxygen-requiring version of cellular respiration. Aerobic respiration consists of four major stages: glycolysis, pyruvate oxidation, the citric acid cycle, and oxidative phosphorylation. The third major stage, the citric acid cycle, is also known as the Krebs cycle or tricarboxylic acid (TCA) cycle.
92.9K
Production of Organic Acids01:25

Production of Organic Acids

Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
105