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A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis
Published on: January 27, 2021
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Salmonella enterica serovar enteritidis modulates intestinal epithelial miR-128 levels to decrease macrophage
Tianfu Zhang1, Jianxiong Yu1, Yaqin Zhang1
1Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University School of Life Sciences, China.
The Journal of Infectious Diseases
|January 14, 2014
Summary
Salmonella infection increases microRNA 128 (miR-128) in intestinal cells, reducing macrophage recruitment and aiding bacterial escape. Inhibiting miR-128 boosts macrophage response and combats Salmonella infection.
Area of Science:
- Microbiology
- Immunology
- Molecular Biology
Background:
- The mechanisms by which virulent Salmonella evades macrophage clearance are not fully understood.
- This study reveals a novel pathway for Salmonella evasion within host cells.
Purpose of the Study:
- To identify key microRNAs regulating Salmonella-host cell interactions.
- To elucidate the role of microRNA 128 (miR-128) in Salmonella pathogenesis.
Main Methods:
- Screening of microRNAs using microarray and quantitative real-time PCR.
- Validation of miR-128 targets with luciferase reporter and Western blot assays.
- In vivo studies using intestinal epithelial cells and a mouse infection model.
Main Results:
- Salmonella infection upregulated miR-128 in intestinal epithelial cells and mouse tissues.
- miR-128 targets Macrophage Colony-Stimulating Factor (M-CSF), reducing its secretion.
- Reduced M-CSF impaired macrophage recruitment, facilitating Salmonella survival.
- Salmonella secreted factors induced miR-128 via the p53 pathway.
- Inhibition of miR-128 enhanced macrophage recruitment and reduced bacterial load in mice.
Conclusions:
- Salmonella upregulates intestinal epithelial miR-128.
- This leads to decreased M-CSF secretion and impaired M-CSF-induced macrophage recruitment.
- Targeting miR-128 represents a potential therapeutic strategy against Salmonella infection.
