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Apoptotic pathway induced by diallyl trisulfide in pancreatic cancer cells

Hong-Bing Ma1, Shan Huang1, Xiao-Ran Yin1

  • 1Hong-Bing Ma, Shan Huang, Xiao-Ran Yin, Yang Zhang, Department of Radiotherapy of Tumor Hospital, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China.

World Journal of Gastroenterology
|January 14, 2014
PubMed
Abstract

Insights

Diallyl trisulfide (DATS), a compound from garlic, effectively suppresses pancreatic cancer cell viability and triggers apoptosis. This study reveals DATS

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Pancreatic cancer remains a significant health challenge with limited effective treatments.
  • Organosulfur compounds, like diallyl trisulfide (DATS) found in garlic, show potential anti-cancer properties.
  • Understanding DATS' mechanisms in pancreatic cancer is crucial for developing new therapeutic strategies.

Purpose of the Study:

  • To investigate the anti-cancer effects of diallyl trisulfide (DATS) on human pancreatic cancer cells.
  • To elucidate the molecular mechanisms underlying DATS-induced apoptosis and cell cycle arrest in pancreatic cancer.
  • To compare the effects of DATS on cancer cells versus normal pancreatic epithelial cells.

Main Methods:

  • Human pancreatic cancer cells (Capan-2) and normal pancreatic epithelial cells (H6C7) were cultured.
  • Cell viability was assessed using the methyl thiazolyl tetrazolium assay.
  • Apoptosis, cell cycle, and protein expression (Western blot, immunofluorescence, qPCR) were analyzed.

Main Results:

  • DATS significantly suppressed pancreatic cancer cell viability and induced apoptosis, particularly in Capan-2 cells.
  • DATS caused cell cycle arrest at the G2/M phase, correlating with altered cyclin B1, p21, and cyclin D1 levels.
  • DATS modulated key apoptosis-related proteins, including upregulation of Fas, p53, and Bax, and downregulation of Bcl-2 and Akt.

Conclusions:

  • Diallyl trisulfide (DATS) demonstrates potent apoptosis-inducing effects on pancreatic cancer cells.
  • DATS affects both cancer and normal pancreatic cells, with a more pronounced apoptotic effect on cancer cells.
  • DATS-mediated cell cycle inhibition and apoptosis involve the modulation of critical cell cycle and apoptosis regulatory proteins.
Keywords:
ApoptosisCell cycleDiallyl trisulfidePancreatic cancerp53

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