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Updated: May 4, 2026

High-throughput Saccharification Assay for Lignocellulosic Materials
Published on: July 3, 2011
Optimization of enzymatic saccharification of microwave pretreated sugarcane tops through response surface
Devendra Prasad Maurya1, Siddharth Vats1, Sudheer Rai1
1Motilal Nehru National Institute of Technology, Allahabad 211 004, India.
Abstract:
The optimization of biomass loading enzyme loading, surfactant concentration and incubation time, using response surface methodology (RSM) and Box Behnken design for enzymatic saccharification of sugarcane tops (SCT) for maximum recovery of fermentable sugars using crude cellulases, resulted in 90.24% saccharification efficiency. Maximum saccharification yield of 0.376 g/g glucose as substrate for ethanol production was observed at optimal conditions of 10% biomass loading (pretreated), 100FPU/g of cellulase loading, 0.04% (w/w) surfactant concentration and 72 h of incubation time.
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