Related Experiment Videos
Human myelin DM-20 proteolipid protein deletion defined by cDNA sequence
Biochemical and Biophysical Research Communications
|July 31, 1987
Summary
Researchers identified a human retinal cDNA clone, pDM-20, encoding a protein similar to myelin proteolipid protein (PLP). This suggests PLP and DM-20 arise from the same gene through alternative splicing.
Area of Science:
- Neuroscience
- Molecular Biology
- Genetics
Background:
- Central Nervous System (CNS) myelin contains major proteolipid proteins.
- Myelin proteolipid protein (PLP) is a key component of CNS myelin.
Purpose of the Study:
- To isolate and characterize cDNA clones related to human myelin proteolipid protein (PLP).
- To investigate the relationship between PLP and the DM-20 protein.
Main Methods:
- A human retinal cDNA library was screened using a PLP cDNA probe.
- A 1kb cDNA clone, pDM-20, was isolated and sequenced.
Main Results:
- The isolated clone pDM-20 contains the coding sequence for PLP, lacking 105 base pairs encoding amino acids 117-151.
- This deletion corresponds to the size of the DM-20 protein, another major CNS myelin proteolipid.
- Sequence homology suggests alternative splicing from a single PLP gene generates both PLP and DM-20 transcripts.
Conclusions:
- The PLP and DM-20 transcripts are likely derived from a single PLP gene via alternative splicing.
- This finding provides molecular insight into the generation of different myelin proteolipid isoforms.