Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Diels–Alder Reaction Forming Bridged Bicyclic Products: Stereochemistry01:29

Diels–Alder Reaction Forming Bridged Bicyclic Products: Stereochemistry

4.3K
Diels–Alder reactions between cyclic dienes locked in an s-cis configuration and dienophiles yield bridged bicyclic products.
4.3K
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

1.3K
When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
1.3K
Stability of Conjugated Dienes01:28

Stability of Conjugated Dienes

3.4K
Introduction
A comparison of the enthalpies of hydrogenation of dienes reveals that conjugated dienes release less heat on hydrogenation, rendering them more stable than their nonconjugated analogs.
3.4K
Preparation of Diols and Pinacol Rearrangement01:57

Preparation of Diols and Pinacol Rearrangement

3.2K
Compounds bearing two hydroxyl groups are known as diols. When the hydroxyl groups are located on adjacent carbon atoms, the diols are called vicinal diols or glycols. Under acidic conditions, vicinal diols undergo a specific reaction called pinacol rearrangement.
The reaction begins with transferring a proton from the acid catalyst to one of the hydroxyl groups, producing an oxonium ion.
3.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mortality trends and disparities in older U.S. adults with atrial fibrillation and COPD: a 1999-2020 CDC WONDER analysis with forecast to 2030.

Frontiers in cardiovascular medicine·2026
Same author

SynCAR platform for capturing neuronal synaptic proteopathic seeds.

iScience·2026
Same author

Evaluation of Immunoprotective Effects of DNA Vaccine Based on <i>Eimeria maxima</i> EF-1α Antigen and Chicken XCL1 Chemokine.

Animals : an open access journal from MDPI·2026
Same author

Latent profile analysis of kinesiophobia during the out-of-hospital early rehabilitation phase after PCI in patients with CHD.

Scientific reports·2026
Same author

Liquid photonic-molecule microlasers for ultrasensitive biosensing.

Nature communications·2026
Same author

The neuronal ALAS2/5-ala axis mitigates chemotherapy-induced neurotoxicity via the BACH1/NRF2 pathway.

British journal of pharmacology·2026

Related Experiment Video

Updated: May 4, 2026

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
07:59

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

11.9K

Diterpenoids from Isodon sculponeatus.

Hua-Yi Jiang1, Wei-Guang Wang2, Min Zhou1

  • 1State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, People's Republic of China; University of the Chinese Academy of Sciences, Beijing 100049, People's Republic of China.

Fitoterapia
|January 15, 2014
PubMed
Summary
This summary is machine-generated.

Researchers identified six new kauranoid compounds from Isodon sculponeatus. One compound showed weak cytotoxic effects and inhibited nitric oxide production in immune cells.

Keywords:
Anti-inflammatory activityBisjaponin A (PubChem CID: 24895695)CytotoxicityDiterpenoidsHebeiabinin B (PubChem CID: 16112773)Isodon sculponeatusLushanrubescensin J (PubChem CID: 11297111)Sculponeatin N (PubChem CID: 42643119)X-ray diffraction

More Related Videos

Author Spotlight: Discovering New Alkaloids in Plants with Advanced Mass Spectrometry Techniques
09:36

Author Spotlight: Discovering New Alkaloids in Plants with Advanced Mass Spectrometry Techniques

Published on: March 8, 2024

1.6K
Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
08:56

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Published on: August 16, 2018

18.3K

Related Experiment Videos

Last Updated: May 4, 2026

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
07:59

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

11.9K
Author Spotlight: Discovering New Alkaloids in Plants with Advanced Mass Spectrometry Techniques
09:36

Author Spotlight: Discovering New Alkaloids in Plants with Advanced Mass Spectrometry Techniques

Published on: March 8, 2024

1.6K
Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
08:56

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Published on: August 16, 2018

18.3K

Area of Science:

  • Natural Product Chemistry
  • Medicinal Chemistry
  • Pharmacology

Background:

  • Isodon sculponeatus is a plant species known for its potential medicinal properties.
  • Diterpenoids, particularly ent-kauranoids, are a class of natural products with diverse biological activities.

Purpose of the Study:

  • To conduct a phytochemical investigation of the aerial parts of Isodon sculponeatus.
  • To isolate and characterize new and known diterpenoid compounds.
  • To evaluate the cytotoxic and anti-inflammatory potential of the isolated compounds.

Main Methods:

  • Extraction and isolation of compounds from plant material.
  • Structure elucidation using extensive spectroscopic analysis (NMR, MS).
  • Determination of absolute configuration via single crystal X-ray diffraction.
  • In vitro assays for cytotoxicity and nitric oxide inhibition.

Main Results:

  • Six new 7,20-epoxy-ent-kauranoids, named sculponins U-Z (compounds 1-6), were isolated.
  • Eleven known diterpenoids (compounds 7-17) were also identified.
  • Compound 5 demonstrated weak cytotoxic activity against HL-60, SMMC-7721, MCF-7, and SW-480 cancer cell lines.
  • Compound 5 inhibited nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW264.7 cells with an IC50 value of 13.8 μM.

Conclusions:

  • The phytochemical analysis of Isodon sculponeatus yielded novel ent-kauranoid diterpenoids.
  • Sculponin U-Z represent new chemical entities with potential pharmacological applications.
  • Compound 5 exhibits moderate anti-inflammatory and weak cytotoxic properties, warranting further investigation.