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Single- and multi-channel currents recorded with patch electrodes in mouse eggs.

A Peres, G Mostacciuolo

    Cell Biology International Reports
    |June 1, 1987
    PubMed
    Summary
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    Zona-free mouse eggs reveal voltage-dependent calcium channels. These channels, crucial for egg function, exhibit low conductance and are densely located on the egg surface, identified via patch recording techniques.

    Area of Science:

    • Cellular Electrophysiology
    • Reproductive Biology
    • Ion Channel Physiology

    Background:

    • Zona-free mouse eggs are suitable for patch and whole-cell recording.
    • Spontaneous channel openings and inward currents were observed in some patches at resting potential.

    Purpose of the Study:

    • To investigate the properties of voltage-dependent calcium currents in mouse eggs.
    • To characterize the conductance, kinetics, and localization of calcium channels in the mouse oocyte.

    Main Methods:

    • Patch-clamp recording techniques (whole-cell and single-channel) were applied to zona-free mouse eggs.
    • Eggs were subjected to voltage-clamp protocols to analyze calcium currents.

    Main Results:

    • Single channel currents of 1.5 pA were inwardly directed at resting potential.

    Related Experiment Videos

  • At -50 mV, two channel populations were observed, with mean open times between 4-7 msec.
  • Microscopic currents resembling whole-cell Ca2+ currents were detected during depolarization.

    • Conclusions:

    • The voltage-dependent Ca2+ current in mouse eggs flows through low-conductance channels.
    • These channels are likely concentrated in specific high-density regions on the egg surface.