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Plasma microRNA profiling of pediatric patients with immune thrombocytopenic purpura
Ali Bay1, Enes Coskun, Serdar Oztuzcu
1aDivision of Pediatric Hematology, Department of Pediatrics, School of Medicine bDepartment of Pediatrics cDepartment of Medical Biology, Gaziantep University, Gaziantep, Turkey.
Abstract:
Immune thrombocytopenic purpura (ITP) is a commonly acquired autoimmune bleeding disorder in children. MicroRNAs (miRNAs) are small RNAs which are found in cells and circulation, and play a role in protein synthesis and regulation. In this study, we aimed to determine a biomarker for childhood ITP comparing the plasma miRNA levels of children having ITP with healthy children. A total of 86 patients with ITP and 56 healthy children followed up by the Department of Pediatric Hematology and Oncology in University of Gaziantep since July 2011 were enrolled in the study. The 86 patients with ITP were evaluated in two groups as 43 acute ITP (aITP) and 43 chronic ITP (cITP) patients. Plasma expression levels of 379 miRNAs were investigated by RT-PCR (quantitative RT-PCR) technique and they were compared between aITP, cITP, and control groups. For all miRNAs, the average of raw quantification cycle values of three groups separately in the analysis chip was accepted as the reference gene value, and normalization was done according to this value. Statistically significant differences were detected in seven miRNAs (miR-302c-3p, miR-483-5p, miR-410, miR-544a, miR-302a-3p, miR-223-3p, and miR-597) investigated between the groups with respect to the expression levels. The expression rates were found to be over 95% in miR-302c-3p and miR-483-5p, over 75% in miR-410, and over 40% in miR-544, miR-302a-3p, and miR-223-3p in all three groups. The detection of significant differences between plasma miRNA levels of aITP and cITP patients and healthy children may provide useful information in the prediction of the course of disease, determination of disease etiopathogenesis, and the development of new therapeutic modalities.
Insights
This study identified seven specific microRNAs (miRNAs) in children with immune thrombocytopenic purpura (ITP) that differ from healthy children. These findings may help predict disease course and develop new therapies for childhood ITP.
Area of Science:
- Pediatric Hematology
- Autoimmune Disorders
- Molecular Biology
Background:
- Immune thrombocytopenic purpura (ITP) is a common autoimmune bleeding disorder in children.
- MicroRNAs (miRNAs) are small regulatory RNAs involved in gene expression and cellular processes.
- Identifying biomarkers for ITP is crucial for diagnosis and management.
Purpose of the Study:
- To identify potential plasma microRNA (miRNA) biomarkers for childhood immune thrombocytopenic purpura (ITP).
- To compare miRNA expression profiles between children with acute ITP (aITP), chronic ITP (cITP), and healthy controls.
- To investigate the role of specific miRNAs in the pathogenesis of childhood ITP.
Main Methods:
- Quantitative reverse transcription polymerase chain reaction (RT-PCR) was used to analyze plasma miRNA expression levels.
- Expression of 379 miRNAs was investigated in 86 ITP patients (43 acute, 43 chronic) and 56 healthy children.
- Data normalization was performed using reference gene values derived from quantification cycle values.
Main Results:
- Statistically significant differences in the expression levels of seven miRNAs (miR-302c-3p, miR-483-5p, miR-410, miR-544a, miR-302a-3p, miR-223-3p, and miR-597) were detected between the groups.
- High expression rates (>95%) were observed for miR-302c-3p and miR-483-5p across all groups.
- Significant differences in plasma miRNA levels were found between ITP patients and healthy children.
Conclusions:
- Plasma miRNA expression profiles differ significantly between children with ITP and healthy controls.
- The identified miRNAs may serve as potential biomarkers for predicting ITP disease course.
- These findings contribute to understanding ITP etiopathogenesis and may inform the development of novel therapeutic strategies.
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