Interaction of dichloromethane (methylene chloride) with the nitrous oxide reductase from Wolinella succinogenes
1Department of Biochemistry, Brandeis University, 02254, Waltham, MA, USA.
Abstract:
Nitrous oxide reductase from Wolinella succinogenes was tested for benzyl viologen cation (BV(+))-chlorinated methane oxidoreductase activity, using di-, tri- and tetra-chloromethanes, and for the inhibition of BV(+)-N2O oxidoreductase activity by these chloromethanes. No BV(+)-chlorinated methane oxidoreductase activity was detected. Any such activity, if it exists, must be less than 0.1% of the BV(+)-N2O oxidoreductase activity of the enzyme. Inhibition of the BV(+)-N2O oxidoreductase activity by dichloromethane was detected and was apparently reversible and non-competitive, as is the case with the small metal-ligand type inhibitors of the enzyme (e.g. acettlene, azide, cyanide and carbon monoxide). Trichloromethane was a weaker inhibitor and inhibition was not detected with tetrachloromethane.
Related Concept Videos
02:14SN2 Reaction: Kinetics
In a chemical reaction, a relationship exists between the concentration of reactants and the rate at which the reaction proceeds. The study to measure this relationship is known as the kinetics of a chemical reaction. Kinetic studies are used to deduce the rate law of a chemical reaction, which provides information about the species involved during the transition state of the rate-determining step. Thus, kinetic studies help to derive the mechanism of a...
02:34Nucleophilic Substitution Reactions
In 1896, the German chemist Paul Walden discovered that he could interconvert pure enantiomeric (+) and (-) malic acids through a series of reactions. This conversion suggested the involvement of optical inversion during the substitution reaction. Further, in 1930, Sir Christopher Ingold described for the first time two different forms of nucleophilic substitution reactions, which are known as SN1 (nucleophilic substitution unimolecular) and SN2 (nucleophilic substitution...
01:33Carboxylic Acids to Methylesters: Alkylation using Diazomethane
01:09Aldehydes and Ketones to Alkanes: Wolff–Kishner Reduction
01:27Radical Substitution: Halogenation of Alkanes and Alkyl Substituents
In the initiation step of the reaction, the chlorine molecule undergoes homolytic cleavage in the presence of light or heat, forming two highly reactive chlorine radicals. Propagation occurs in two...
00:55Nitriles to Amines: LiAlH4 Reduction
As shown below, the mechanism involves three steps. Firstly, the hydride ion acting as a nucleophile attacks the nitrile carbon to form an anion. In the second step, a second equivalent of the hydride ion attacks the anion to...