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Related Concept Videos

Glucose Absorption Into the Small Intestine01:26

Glucose Absorption Into the Small Intestine

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Complex carbohydrates consumed cannot be absorbed into the small intestine in their original form. First, they must be hydrolyzed to a monosaccharide form such as glucose or galactose. These monosaccharides are then transported across the intestinal membrane and into the blood via transcellular transport. The intestinal epithelial cells allow the movement of these monosaccharides with a defined 'entry' through membrane transporter proteins present on their apical membrane and...
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Glucose transporters facilitate the transport of glucose across the cell membrane. In addition to glucose, some glucose transporters can also aid the movement of other hexoses such as fructose, mannose, and galactose.
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Plasma membranes have integral transmembrane proteins involved in facilitated transport. These proteins are collectively referred to as transport proteins, and they function as either channels for the material or as carriers themselves. Channel proteins have hydrophilic domains exposed to the intracellular and extracellular fluids and a hydrophilic channel through their core that provides a hydrated opening for solutes to pass through the membrane layers. Passage through the channel allows...
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Glucose uptake by Cellulomonas fimi.

S Khanna1

  • 1Microbiology and Molecular Genetics Unit, Tata Energy Research Institute, 158 Jor Bagh, 110 003, New Delhi, India.

World Journal of Microbiology & Biotechnology
|January 15, 2014
PubMed
Summary

Cellulomonas fimi actively transports glucose via a specific system. Glucose uptake is enhanced aerobically and inhibited by ionophores and cellobiose, indicating a unique transport mechanism.

Area of Science:

  • Microbiology
  • Cellular Physiology
  • Biochemistry

Background:

  • Cellulomonas fimi is a facultative anaerobe with known metabolic capabilities.
  • Understanding nutrient transport is crucial for characterizing microbial physiology.

Purpose of the Study:

  • To investigate the mechanism of glucose transport in Cellulomonas fimi.
  • To identify factors influencing glucose uptake rates and specificity.

Main Methods:

  • Studying glucose uptake in whole-cell suspensions under varying conditions (aerobic, anaerobic).
  • Utilizing specific inhibitors of electron transport, ATP synthesis, and ionophores.
  • Assessing the effect of different sugars and growth media on glucose transport.

Main Results:

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  • Glucose uptake was twofold higher under aerobic conditions compared to anaerobic.
  • Uptake was significantly inhibited by electron transport inhibitors, ATP synthesis inhibitors, and ionophores.
  • Cellobiose acted as a non-competitive inhibitor, and cells grown on cellobiose showed reduced glucose transport rates.
  • 2-deoxyglucose did not inhibit glucose uptake, suggesting high specificity.

Conclusions:

  • Cellulomonas fimi possesses a highly specific active transport system for glucose.
  • Environmental conditions and substrate availability influence glucose transport efficiency.