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Related Experiment Video

Updated: May 4, 2026

Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism

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Published on: December 18, 2013

Mild temperature "stress" and callose synthesis.

M M Smith¹, M E McCully

¹Department of Biology, Carleton University, Colonel By Drive, K1S 5B6, Ottawa, Ont., Canada.

|January 15, 2014

Summary

Temperature significantly impacts callose deposition in plants. Cooler temperatures (20°C) increased callose in corn, soybean, and mung bean, while warmer temperatures (26°C) increased it in pea and tomato seedlings.

Area of Science:

Plant physiology
Cell biology
Biochemistry

Background:

Callose (β-1,3-glucans) plays crucial roles in plant cell wall structure and defense.
Temperature is a key environmental factor influencing plant growth and development.
Understanding callose dynamics under varying temperatures is vital for plant science.

Purpose of the Study:

To investigate the effect of different temperatures on callose deposition in various crop and vegetable seedlings.
To examine temperature-dependent variations in aniline-blue fluorescence as an indicator of callose.

Main Methods:

Seedlings of Zea mays, Sorghum vulgare, Pisum sativum, Phaseolus aureus, Glycine max, and Lycopersicum esculentum were cultivated at 20°C and 26°C.
Plant tissues were fixed in glutaraldehyde.

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Aniline-blue-induced fluorescence was analyzed to quantify callose presence.

Main Results:

Zea mays, Glycine max, and Phaseolus aureus exhibited higher aniline-blue fluorescence at 20°C compared to 26°C.
Pisum sativum and Lycopersicum esculentum showed increased fluorescence at 26°C versus 20°C.
In Zea mays, fluorescent material was prominent in elongating cells near the shoot apex and root cap, associated with pitfields.

Conclusions:

Plant species exhibit differential responses in callose deposition to temperature variations.
Temperature influences the localization and abundance of callose in specific cell types and structures.
These findings contribute to understanding plant thermotolerance and cell wall dynamics.