Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bioreactor Controls-II01:18

Bioreactor Controls-II

76
In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the...
76
Bioreactor Controls-III01:22

Bioreactor Controls-III

67
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
67
Microbial Interactions: Mutualism01:25

Microbial Interactions: Mutualism

80
Mutualism is a symbiotic interaction in which all participating organisms benefit. These relationships can be obligate or facultative and are fundamental to ecosystem functions across diverse biological systems.Plant–Fungi MutualismOne well-known example is the association between plant roots and mycorrhizal fungi, such as Rhizophagus species. The fungal hyphae penetrate the root hairs and the epidermis, forming an extensive hyphal network that establishes a symbiotic association. Through...
80
Production of Organic Acids01:25

Production of Organic Acids

105
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
105
Microbial Fermentation01:23

Microbial Fermentation

1.8K
Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
1.8K
Fates of Pyruvate01:20

Fates of Pyruvate

9.1K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
9.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lewis Acid Site Engineering in Chromite Spinels Orchestrated Surface Reconstruction and Surpasses RuO<sub>2</sub> in Oxygen Evolution.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

Enhanced Electrocatalytic Activity of Ecofriendly and Earth-Abundant (Zn,Cu)Fe<sub>2</sub>O<sub>4</sub> + CuO Nanocomposites for Water Splitting.

ACS applied materials & interfaces·2025
Same author

2D MoS<sub>2</sub> for Next-Generation Electronics and Optoelectronics: From Material Properties to Manufacturing Challenges and Future Prospects.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

Unveiling the potential of novel Metschnikowia yeast biosurfactants: triggering oxidative stress for promising antifungal and anticancer activity.

Microbial cell factories·2024
Same author

Nanoengineered pure Fe in a citrate matrix (Fe-CIT) with significant and tunable magnetic properties.

Nanotechnology·2024
Same author

One-Step Synthesis and Operando Electrochemical Impedance Spectroscopic Characterization of Heterostructured MoP-Mo<sub>2</sub>N Electrocatalysts for Stable Hydrogen Evolution Reaction.

ACS applied materials & interfaces·2024

Related Experiment Video

Updated: May 4, 2026

Assembly and Quantification of Co-Cultures Combining Heterotrophic Yeast with Phototrophic Sugar-Secreting Cyanobacteria
05:44

Assembly and Quantification of Co-Cultures Combining Heterotrophic Yeast with Phototrophic Sugar-Secreting Cyanobacteria

Published on: December 27, 2024

1.7K

H2 production by mixed cultures.

C Sasikala1, C V Ramana, G S Prasad

  • 1, .

World Journal of Microbiology & Biotechnology
|January 15, 2014
PubMed
Summary

Hydrogen (H2) production from glucose was optimized using a mixed culture of Rhodobacter sphaeroides and Pseudomonas fluorescens. Immobilization further enhanced H2 yield, while other combinations showed inhibition.

Area of Science:

  • Microbiology
  • Biotechnology
  • Sustainable Energy

Background:

  • Microbial hydrogen production is a promising renewable energy source.
  • Different bacterial types, including phototrophic and heterotrophic, exhibit varying H2 generation capabilities.
  • Optimizing microbial consortia can enhance biohydrogen production efficiency.

Purpose of the Study:

  • To evaluate H2 production from glucose using individual and mixed cultures of Rhodobacter sphaeroides, Synechococcus cedrorum, and Pseudomonas fluorescens.
  • To determine the optimal bacterial combination for maximal H2 yield.
  • To assess the effect of bacterial immobilization on H2 production.

Main Methods:

  • Culturing of Rhodobacter sphaeroides, Synechococcus cedrorum, and Pseudomonas fluorescens individually and in various mixed combinations.

More Related Videos

Author Spotlight: Unraveling the Mysteries of Terrestrial Anaerobic Microorganisms in Uncharted Environments by In Situ Culturing
07:56

Author Spotlight: Unraveling the Mysteries of Terrestrial Anaerobic Microorganisms in Uncharted Environments by In Situ Culturing

Published on: January 12, 2024

1.7K
Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions
06:17

Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions

Published on: August 15, 2019

31.3K

Related Experiment Videos

Last Updated: May 4, 2026

Assembly and Quantification of Co-Cultures Combining Heterotrophic Yeast with Phototrophic Sugar-Secreting Cyanobacteria
05:44

Assembly and Quantification of Co-Cultures Combining Heterotrophic Yeast with Phototrophic Sugar-Secreting Cyanobacteria

Published on: December 27, 2024

1.7K
Author Spotlight: Unraveling the Mysteries of Terrestrial Anaerobic Microorganisms in Uncharted Environments by In Situ Culturing
07:56

Author Spotlight: Unraveling the Mysteries of Terrestrial Anaerobic Microorganisms in Uncharted Environments by In Situ Culturing

Published on: January 12, 2024

1.7K
Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions
06:17

Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions

Published on: August 15, 2019

31.3K
  • Incubation under light conditions to facilitate phototrophic H2 production.
  • Immobilization of bacteria in alginate gel to evaluate its impact on H2 yield.

    • Main Results:

    • Maximal H2 production was achieved with a mixed culture of Rhodobacter sphaeroides and Pseudomonas fluorescens.
    • Immobilization of this mixed culture in alginate gel further enhanced H2 production.
    • Inhibition of H2 production was observed in mixtures of Synechococcus cedrorum and Pseudomonas fluorescens, and in a co-culture of all three organisms.

    Conclusions:

    • A synergistic interaction between Rhodobacter sphaeroides and Pseudomonas fluorescens significantly boosts H2 production from glucose.
    • Alginate immobilization is an effective strategy to enhance microbial H2 yield.
    • Specific microbial combinations can lead to inhibitory effects, underscoring the importance of careful consortia design for biohydrogen production.