Related Experiment Video
Updated: May 4, 2026

Differentiated Mouse Adipocytes in Primary Culture: A Model of Insulin Resistance
Published on: February 17, 2023
Subcutaneous adipose tissue insulin resistance is associated with visceral adiposity in postmenopausal women
Beret A Casey1, Wendy M Kohrt, Robert S Schwartz
1Division of Endocrinology, Diabetes and Metabolism, Department of Medicine, University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA.
Objective:
Whole body and subcutaneous adipose tissue (SAT) insulin resistance association with regional fat mass (FM) was determined.
Methods:
Postmenopausal women (mean ± SD; age 56 ± 4 years, n = 25) who were overweight or obese (BMI 29.9 ± 5.1 kg/m(2) ) were studied. Whole body and regional FM were measured by dual-energy X-ray absorptiometry (DXA) and computed tomography (CT). Women were studied during basal and insulin-stimulated (3-stage euglycemic clamp) conditions. Whole-body lipolysis was assessed by [(2) H5 ]-glycerol rate of appearance and abdominal and femoral SAT lipolysis by interstitial glycerol (microdialysis).
Results:
Whole body insulin resistance in skeletal muscle (insulin-stimulated glucose disposal) and adipose tissue (insulin-suppressed lipolysis) were independently related to trunk FM (r = -0.336 and 0.484, respectively), but not leg FM (r = -0.142 and -0.148, respectively). Local antilipolytic insulin resistance in abdominal, but not femoral, SAT was positively related to trunk FM (r = 0.552) and visceral FM (r = 0.511) but not related to leg FM (r = -0.289). Whole body and abdominal, but not femoral, adipose tissue insulin sensitivity were strongly related to skeletal muscle insulin sensitivity (r = -0.727 and -0.674, respectively).
Conclusions:
The association of SAT insulin sensitivity (lipolysis) with adiposity and skeletal muscle insulin sensitivity was specific to the abdominal region.
More Related Videos
06:22Isolation of Viable Adipocytes and Stromal Vascular Fraction from Human Visceral Adipose Tissue Suitable for RNA Analysis and Macrophage Phenotyping
Published on: October 27, 2020
10:37Author Spotlight: Decellularization-Based Quantification of Skeletal Muscle Fatty Infiltration
Published on: June 9, 2023
Related Concept Videos
Cholesterol: Significance and Regulation
Considering cholesterol and...
Hypodermis
Type II Diabetes II: Pathophysiology
Pharmacokinetics in Obese Patients: Drug Absorption and Distribution
Obesity
Type II Diabetes I: Introduction