Related Experiment Video

Updated: May 4, 2026

Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma
10:02

Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma

Published on: June 2, 2023

2.8K

Purification and properties of β-amylase from Bacillus circulans S31

H S Kwan1, K H So, K Y Chan

  • 1, .

World Journal of Microbiology & Biotechnology
|January 15, 2014
PubMed

Abstract:

A thermotolerant β-amylase was purified from Bacillus circulans S31 isolated from soil in Hong Kong. The purified enzyme has an M r of 64 kDa and was stable at 50°C and pH 7.0 for 30 min. Its K m for starch was 0.9 mg/ml with a V max of 0.3 mg/min. It was not activated by any metal ion although sulphydrys reagents were inhibitory.

More Related Videos

Author Spotlight: Integrating Biochemical Functions of β-Glucanases and Peroxidase Enzymes in Wheat-RWA Interaction
10:26

Author Spotlight: Integrating Biochemical Functions of β-Glucanases and Peroxidase Enzymes in Wheat-RWA Interaction

Published on: July 26, 2024

1.4K
Author Spotlight: Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus
06:45

Author Spotlight: Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus

Published on: June 2, 2023

2.4K

Related Experiment Videos

Last Updated: May 4, 2026

Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma
10:02

Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma

Published on: June 2, 2023

2.8K
Author Spotlight: Integrating Biochemical Functions of β-Glucanases and Peroxidase Enzymes in Wheat-RWA Interaction
10:26

Author Spotlight: Integrating Biochemical Functions of β-Glucanases and Peroxidase Enzymes in Wheat-RWA Interaction

Published on: July 26, 2024

1.4K
Author Spotlight: Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus
06:45

Author Spotlight: Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus

Published on: June 2, 2023

2.4K

Related Concept Videos

Production of Organic Acids01:25

Production of Organic Acids

105
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
105

Articles linked to this work by shared authors, journal, and citation graph.

[Adverse reaction surveillance analysis of domestic human papillomavirus vaccines with different production processes].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2025

Emerging applications of sustainable modified CdO/Ag-CdO NPs for electrochemical sensitive and selective detection of mercury (Hg+) heavy metal.

Scientific reports·2025

Validation and repeatability assessment of the Chinese version of the Ocular Surface Disease Index (OSDI), 5-item dry eye (DEQ-5), and contact lens dry eye questionnaire-8 (CLDEQ-8) questionnaires.

Contact lens & anterior eye : the journal of the British Contact Lens Association·2024

Development of a sustainable and disposable modified Bi-CdFe2O4 electrode for electrochemical sensing of lead (II) and Acetaminophen drug molecule.

Scientific reports·2024

Multicentre study of hospitalised patients with sports- and recreational cycling-related traumatic brain injury in Hong Kong.

Hong Kong medical journal = Xianggang yi xue za zhi·2021

Febrile neutropenia and its associated hospitalization in breast cancer patients on docetaxel-containing regimen: A retrospective cohort study on duration of prophylactic GCSF administration.

Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer·2019

Comparative genomics and biomineralization potential of a native ureolytic Bacillus sp. N₉ for microbially induced calcite precipitation.

World journal of microbiology & biotechnology·2026

Advances in glycerol metabolism comprehension in yeasts: from regulation to metabolic engineering strategies.

World journal of microbiology & biotechnology·2026

A comparative study of composite probiotic yogurt on LPS-induced acute inflammation and immunomodulation in mice.

World journal of microbiology & biotechnology·2026

Host-driven structuring shapes the landscape of phytoplasma diversity across Indian agroecosystems revealed by multilocus phylogenomics.

World journal of microbiology & biotechnology·2026

Potential of pongam oil and karanjin as natural nitrification inhibitors: mechanistic effects on nitrifying microorganisms and soil nitrogen transformation.

World journal of microbiology & biotechnology·2026

Phycoremediation of methylene blue mediated by algal coculture: insights into morpho-physiological and metabolic responses.

World journal of microbiology & biotechnology·2026

Electrical Properties in Biological Materials.

Chemical reviews·2026

Conformational Ensemble and Stabilization of Insulin by a Choline Ionic Liquid Elucidated through Markov State Models.

Journal of chemical information and modeling·2026

Budding yeast Bud3 and Bud4 cooperatively organize septin architecture and support cytokinetic spatial memory.

Cell reports·2026

From Structure to Dynamics: Activation Mechanism of the G Protein-Coupled Bile Acid Receptor 1‑Gs Complex.

ACS omega·2026

Mitochondrial Cristae as Separate Compartments: Linking Organization and Function.

Current issues in molecular biology·2026

Characterising C-X-C Chemokine Receptor 4 Dynamics in the Cell Membrane Using Fluorescence Fluctuation Spectroscopy.

Biomolecules·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies
Jove
Visualize
Contact Us