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Related Experiment Videos

Sodium/proton antiporter in Streptococcus faecalis.

Y Kakinuma

    Journal of Bacteriology
    |September 1, 1987
    PubMed
    Summary

    Streptococcus faecalis expels sodium ions via two pathways. Besides the known ATP-driven pump, a second pathway uses a proton motive force, suggesting a sodium/proton antiporter.

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    Area of Science:

    • Microbiology
    • Cellular Physiology

    Background:

    • Streptococcus faecalis actively transports ions, accumulating potassium and expelling sodium.
    • Previous research identified an ATP-dependent sodium pump responsible for sodium extrusion.

    Purpose of the Study:

    • To investigate the mechanism of sodium extrusion in Streptococcus faecalis.
    • To identify alternative pathways for sodium ion expulsion beyond the ATP-linked pump.

    Main Methods:

    • Assessing sodium extrusion in cells with uninduced sodium ATPase.
    • Observing sodium extrusion under varying pH conditions and glucose availability.
    • Testing the effects of dicyclohexylcarbodiimide and proton-conducting ionophores.
    • Utilizing artificial pH gradients to study energy-depleted cells.

    Main Results:

    • Sodium extrusion occurs even when the sodium ATPase is not induced, indicating an alternative pathway.
    • This alternate pathway requires a proton motive force, dependent on glucose and neutral pH.
    • Inhibition by dicyclohexylcarbodiimide and ionophores, along with observations using pH gradients, supports the involvement of proton movement.

    Conclusions:

    • Streptococcus faecalis possesses two distinct sodium extrusion systems.
    • These systems are an inducible sodium ATPase and a constitutive sodium/proton antiporter.

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