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Related Experiment Videos

Methotrexate-induced changes in rat parotid and submandibular gland function.

R K McBride, C Harper, I A Siegel

    Journal of Dental Research
    |September 1, 1987
    PubMed
    Summary

    Methotrexate (MTX) treatment in rats reduced salivary gland weight and function. This study investigated MTX

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    Area of Science:

    • Biochemistry
    • Physiology
    • Pharmacology

    Background:

    • Methotrexate (MTX) is a chemotherapy agent and immunosuppressant.
    • Salivary glands are crucial for oral health and digestion.
    • Understanding MTX's effects on salivary glands is important for managing side effects.

    Purpose of the Study:

    • To investigate the effects of MTX on rat salivary gland function.
    • To examine changes in gland weight, RNA, and amylase content.
    • To assess the impact of MTX on ion transport in salivary ducts.

    Main Methods:

    • Adult rats received daily intraperitoneal injections of MTX (15 mg/kg) for three consecutive days.
    • Salivary gland weight, RNA, and amylase content were measured.
    • Sodium, chloride, and potassium outputs were analyzed in parotid and submandibular glands.
    • Ductal perfusion was used to study ion transport in submandibular glands.

    Main Results:

    • MTX significantly decreased unstimulated parotid gland weight, RNA, and amylase content compared to controls.
    • Submandibular gland RNA content was also reduced by MTX.
    • Parotid and submandibular gland outputs of sodium and chloride decreased, while potassium output remained unchanged.
    • Ductal perfusion revealed a reduced net efflux of sodium and chloride from the submandibular gland excretory duct.

    Conclusions:

    • MTX administration impairs salivary gland function, affecting weight, protein synthesis, and ion transport.
    • The observed effects on gland weight, RNA, and amylase suggest inhibition of protein synthesis.
    • The precise mechanism underlying MTX-induced alterations in salivary gland ion transport requires further investigation.

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