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In Vivo Proximity Biotinylation for Protein Interaction Studies in Paramecium tetraurelia
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In vivo [35S]-methionine incorporation
Anthony M Esposito1, Terri Goss Kinzy1
1UMDNJ Robert Wood Johnson Medical School, Piscataway, NJ, USA.
Methods in Enzymology
|January 16, 2014
Abstract:
The purpose of this assay is to measure the incorporation of radiolabeled [(35)S]-methionine into newly synthesized proteins in exponentially growing yeast cells. This allows for a quantitative in vivo measurement of total protein synthesis.
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