Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immunocytochemical methods: a technical overview.

J B Matthews

    Journal of Oral Pathology
    |April 1, 1987
    PubMed
    Summary
    This summary is machine-generated.

    This study reviews immunocytochemical techniques, highlighting challenges in maintaining tissue reactivity and antibody specificity. It addresses issues from tissue processing and cross-reacting antibodies to monoclonal antibody lineage non-specificity.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A study on the prevalence of cyathostomin and Anoplocephala perfoliata infections in Italian horses: diagnostic testing and analysis of factors affecting infection risk.

    Journal of equine veterinary science·2026
    Same author

    Clinical predictors of donor antibody titre and correlation with recipient antibody response in a COVID-19 convalescent plasma clinical trial.

    Journal of internal medicine·2020
    Same author

    Immediate and long-term impact of the COVID-19 pandemic on delivery of surgical services.

    The British journal of surgery·2020
    Same author

    Cigarette smoke modifies neutrophil chemotaxis, neutrophil extracellular trap formation and inflammatory response-related gene expression.

    Journal of periodontal research·2018
    Same author

    Pain Control, Glucose Control, and Quality of Life in Patients With Chronic Pancreatitis After Total Pancreatectomy With Islet Autotransplantation: A Preliminary Report.

    Transplantation proceedings·2017
    Same author

    P-glycoproteins play a role in ivermectin resistance in cyathostomins.

    International journal for parasitology. Drugs and drug resistance·2017
    Same journal

    Postmortem pink teeth: in vitro production.

    Journal of oral pathology·1988
    Same journal

    Clinical and chemical properties of a novel mucosal bioadhesive agent.

    Journal of oral pathology·1988
    Same journal

    Multiple myeloma and amyloidosis of the tongue.

    Journal of oral pathology·1988
    Same journal

    Amelogenesis imperfecta, dentinogenesis imperfecta and dentin dysplasia revisited: problems in classification.

    Journal of oral pathology·1988
    Same journal

    The unicystic ameloblastoma: a clinicopathological study of 57 cases.

    Journal of oral pathology·1988
    Same journal

    Histomorphometric analysis of epithelial changes in chemically induced oral mucosal carcinogenesis in rats.

    Journal of oral pathology·1988
    See all related articles

    Area of Science:

    • Immunocytochemistry
    • Biotechnology
    • Histology

    Background:

    • Immunocytochemistry is a vital tool for visualizing cellular components.
    • Ensuring accurate results requires addressing technical challenges.
    • Antibody specificity and tissue integrity are critical for reliable outcomes.

    Purpose of the Study:

    • To describe common immunocytochemical techniques and their applications.
    • To discuss critical issues affecting immunocytochemical analysis.
    • To provide insights into optimizing immunocytochemical protocols.

    Main Methods:

    • Literature review of immunocytochemical techniques.
    • Analysis of factors impacting tissue immunoreactivity.
    • Evaluation of antibody specificity challenges.

    Related Experiment Videos

    Main Results:

    • Common immunocytochemical methods and applications are detailed.
    • Factors compromising tissue immunoreactivity are identified.
    • Issues with antibody specificity, including cross-reactivity and lineage non-specificity, are discussed.

    Conclusions:

    • Understanding technical limitations is key to accurate immunocytochemistry.
    • Careful tissue processing and antibody selection are essential.
    • Further research may improve the reliability of immunocytochemical assays.