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Structure and function of sodium channel.

M Noda, S Numa

    Journal of Receptor Research
    |January 1, 1987
    PubMed
    Summary
    This summary is machine-generated.

    Researchers have determined the structures of electric eel and rat brain sodium channels using cDNA cloning. These findings reveal the channel

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    Area of Science:

    • Molecular Biology
    • Neuroscience
    • Biochemistry

    Background:

    • Sodium channels are crucial for nerve impulse transmission.
    • Understanding their structure is key to deciphering neuronal function.

    Purpose of the Study:

    • To elucidate the primary structures of sodium channels from Electrophorus electroplax and rat brain.
    • To investigate the molecular basis of sodium channel function and gating.

    Main Methods:

    • cDNA cloning and sequencing were employed to determine primary structures.
    • Messenger RNA (mRNA) injection into Xenopus oocytes was used to assess functional expression.

    Main Results:

    • The primary structures of Electrophorus electroplax and rat brain sodium channels were deduced.

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  • Sodium channel molecules exhibit four internal homologous repeats, suggesting a pseudosymmetric transmembrane arrangement.
  • Functional sodium channels were expressed in Xenopus oocytes from rat brain sodium channel cDNAs.

    • Conclusions:

    • The deduced structures provide insights into the transmembrane topology of sodium channels.
    • The findings offer a basis for understanding the voltage-dependent gating mechanisms of sodium channels.