Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ionic channels and membrane hyperpolarization in human macrophages.

C Ince, B Van Duijn, D L Ypey

    The Journal of Membrane Biology
    |January 1, 1987
    PubMed
    Summary

    Microelectrode impalement of human macrophages triggers a membrane potential hyperpolarizing response (HR). This response is mediated by high-conductance, voltage-dependent potassium channels activated by calcium.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The polyunsaturated fatty acid and oxylipin plasma signature of aneurysmal subarachnoid haemorrhage, case-control study.

    Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics·2025
    Same author

    Chlorophyll Fluorescence, Oxygen Consumption Rates and Germination of Green Soybean Seeds Produced Under Heat-Drought Stress.

    Plant direct·2025
    Same author

    Alleviation of Post-sepsis Ischaemia by Drag-Reducing Polymers.

    Advances in experimental medicine and biology·2024
    Same author

    Handheld vital microscopy for the identification of microcirculatory alterations in cervical intraepithelial neoplasia and cervical cancer.

    Microvascular research·2023
    Same author

    The future of intensive care: the study of the microcirculation will help to guide our therapies.

    Critical care (London, England)·2023
    Same author

    Real-time observation of microcirculatory leukocytes in patients undergoing major liver resection.

    Scientific reports·2021

    Area of Science:

    • Cellular Electrophysiology
    • Ion Channel Physiology
    • Immunology

    Background:

    • Human macrophages exhibit a transient hyperpolarizing response (HR) upon microelectrode impalement.
    • The underlying ionic mechanisms of this HR are not fully understood.

    Purpose of the Study:

    • To investigate the ionic basis of the microelectrode-induced HR in human macrophages.
    • To identify the specific ion channels involved in this cellular response.

    Main Methods:

    • Patch-clamp technique in cell-attached configuration on intact human macrophages.
    • Microelectrode impalement to evoke the hyperpolarizing response.
    • Varying extracellular ion concentrations (K+, Na+, Cl-) and using specific blockers (quinine, Mg2+-EGTA).

    Related Experiment Videos

    Main Results:

    • The HR is dependent on extracellular K+ but not Na+ or Cl-.
    • The response is blocked by quinine and Mg2+-EGTA, suggesting K+(Ca2+) conductance.
    • Patch-clamp identified low and high-conductance voltage-dependent K+ channels.
    • Enhanced activity of high-conductance channels was observed during the HR, distinct from pre-existing channels.

    Conclusions:

    • The hyperpolarizing response in human macrophages is primarily mediated by the activation of high-conductance, voltage-dependent potassium channels.
    • Calcium influx likely plays a role in activating these K+ channels during the HR.
    • These findings elucidate a novel electrophysiological response in macrophages.