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Updated: May 4, 2026
Inducing Acute Lung Injury in Mice by Direct Intratracheal Lipopolysaccharide Instillation
Published on: July 6, 2019
Methyl palmitate attenuates lipopolysaccharide-induced acute lung injury in mice
S Kalemci1, A Zeybek, Y S Intepe
1Departments of Chest Diseases, Faculty of Medicine Mugla University, Mugla, Turkey.
Aim:
The study is aimed to determine the beneficial effects of methyl palmitate (MP) which has antioxidant and anti-inflammatory effects demonstrated on murine model of acute lung injury induced by lipopolysaccharide (LPS).
Materials And Methods:
Forty male BALB/C mice were randomly allocated into four groups (n=10, each): control group, methyl palmitate group (300 mg/kg), LPS group, and methyl palmitate -treated groups. Methyl palmitate or vehicle was given with an intraperitoneal administration 1 h before an intratracheal instillation of LPS (5 mg/kg). The severity of pulmonary injury was evaluated 6 h after LPS challenge. All experimental procedures complied with the requirements of the Animal Care and Ethics Committee of the Adnan Menderes University.
Results:
Methyl palmitate pretreatment significantly attenuated LPS-induced pulmonary histopathologic changes, alveolar hemorrhage, and neutrophil infiltration. Methyl palmitate pretreatment also reduced the concentrations of malondialdehyde in lung tissues.
Conclusions:
This study indicates that methyl palmitate may have a protective effect against LPS-induced acute lung injury, and the potential mechanism of this action may involve the inhibition of NF-κB. activation.
Insights
Methyl palmitate pretreatment protected against acute lung injury caused by lipopolysaccharide (LPS) in mice. This suggests methyl palmitate may be a potential therapeutic agent for lung inflammation.
Area of Science:
- Biomedical Science
- Pharmacology
- Immunology
Background:
- Acute lung injury (ALI) induced by lipopolysaccharide (LPS) is a significant clinical concern.
- Methyl palmitate (MP) exhibits known antioxidant and anti-inflammatory properties.
- Investigating MP's therapeutic potential in ALI models is crucial.
Purpose of the Study:
- To evaluate the protective effects of methyl palmitate (MP) against lipopolysaccharide (LPS)-induced acute lung injury (ALI) in a murine model.
- To explore the potential mechanisms underlying MP's therapeutic actions in ALI.
Main Methods:
- Male BALB/C mice were divided into four groups: control, MP only, LPS only, and MP + LPS.
- MP (300 mg/kg) or vehicle was administered intraperitoneally 1 hour before intratracheal LPS (5 mg/kg) instillation.
- Pulmonary injury was assessed 6 hours post-LPS challenge, evaluating histopathology, hemorrhage, neutrophil infiltration, and malondialdehyde levels.
Main Results:
- Methyl palmitate pretreatment significantly reduced LPS-induced pulmonary histopathological damage.
- MP administration attenuated alveolar hemorrhage and neutrophil infiltration in the lungs.
- Reduced malondialdehyde concentrations in lung tissues were observed following MP pretreatment.
Conclusions:
- Methyl palmitate demonstrates a protective effect against LPS-induced acute lung injury.
- The mechanism may involve the inhibition of nuclear factor-kappa B (NF-κB) activation.
- MP shows promise as a potential therapeutic agent for ALI.
