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Related Concept Videos

Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

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Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
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Notch Signaling Pathway03:14

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The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
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Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
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Related Experiment Video

Updated: May 4, 2026

Application of a Mouse Ligated Peyer&#8217;s Patch Intestinal Loop Assay to Evaluate Bacterial Uptake by M cells
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Mouse and human Notch-1 regulate mucosal immune responses.

D R Mathern1, L E Laitman1, Z Hovhannisyan1

  • 1Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA.

Mucosal Immunology
|January 16, 2014
PubMed
Summary
This summary is machine-generated.

Epithelial Notch-1 is crucial for gut immunity, bridging innate and adaptive responses. It regulates intestinal epithelial cell activation and immune signaling, supporting protective inflammation.

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Area of Science:

  • Immunology
  • Gastroenterology
  • Cellular Signaling

Background:

  • The Notch-1 signaling pathway regulates epithelial barrier function and is implicated in inflammatory conditions like colitis.
  • Lymphoepithelial crosstalk is vital for maintaining gut homeostasis and responding to disease.
  • The specific role of colonic epithelial Notch-1 in this crosstalk remains to be fully elucidated.

Purpose of the Study:

  • To investigate the function of colonic epithelial Notch-1 in mediating lymphoepithelial crosstalk during health and inflammation.
  • To determine how Notch-1 signaling influences intestinal epithelial cell activation and immune responses in the gut.

Main Methods:

  • Utilized in vivo and in vitro knockdown techniques to reduce Notch-1 expression in the colon.
  • Assessed epithelial cell activation, mucosal chemokine and cytokine secretion, and T-cell responses (including FoxP3+ cells).

Main Results:

  • Epithelial Notch-1 is essential for intestinal epithelial cell activation in both steady-state and inflammatory conditions.
  • Notch-1 signaling modulates the secretion of key immune mediators and influences T-cell populations.
  • Epithelial Notch-1 interacts with nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways to regulate T-cell responses.

Conclusions:

  • Epithelial Notch-1 acts as a critical link between innate and adaptive immunity in the gastrointestinal tract.
  • Notch-1 signaling is indispensable for protective inflammatory responses within the gut epithelium.
  • Targeting epithelial Notch-1 may offer therapeutic potential for inflammatory gut diseases.