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Ex Vivo Infection of Live Tissue with Oncolytic Viruses

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Published on: June 25, 2011

The live vector approach-viruses.

¹Cancer Research Campaign Laboratories, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Wilmslow Rd., M20 9BX, Withington, Manchester, UK.

World Journal of Microbiology & Biotechnology

|January 16, 2014

Summary

This summary is machine-generated.

Recombinant DNA technology enables engineering vaccinia virus (the smallpox vaccine) as a versatile expression system for developing novel vaccines against foreign pathogens. Vaccinia recombinants show promise for animal vaccines and studying cellular immunity.

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Area of Science:

Molecular Biology
Virology
Vaccinology

Background:

Recombinant DNA technology allows expression of foreign genes in viruses and bacteria.
This has spurred the development of live attenuated vaccines expressing protective antigens from pathogens.

Purpose of the Study:

To explore the use of vaccinia virus as a platform for engineering live attenuated vaccines.
To assess the potential of vaccinia recombinants for vaccine development and studying cellular immunity.

Main Methods:

Engineering vaccinia virus (smallpox vaccine) as a recombinant expression system.
Developing and testing vaccinia recombinants expressing foreign antigens in animal models.

Main Results:

Hundreds of vaccinia recombinants have been created, with many demonstrating protective immunity in animals.
Field trials of a vaccinia recombinant expressing rabies virus glycoprotein have shown success.

Conclusions:

Vaccinia virus is a valuable expression system, particularly for studying cellular immunity.
Vaccinia recombinants show significant potential as animal vaccines and for developing new vaccines against infectious diseases.