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Updated: May 4, 2026

Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
Published on: May 30, 2017
Toxigenicity and toxin genes of Vibrio cholerae 01 isolated from an artificial aquatic environment
1Department of Tropical Hygiene, London School of Hygiene and Tropical Medicine, Keppel Street, WC1E 7HT, London, UK.
Abstract:
A simple experiment was carried out to examlne the effect of varlous physicochemical conditions on toxigenicity and toxin genes of Vibrio cholerae 01 lsolated from an artificial aquatic environment. All isolated strains, tested by tissue culture assay, DNA-DNA hybridization and ELISA, were cytotoxic to Vero cells, did not lose their toxin genes and continued to produce cholera toxin. These results are consistent with the hypothesis that V. cholerae can survive in the environment without losing potential pathogenicity.
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