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Updated: May 4, 2026

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry
Published on: March 13, 2014
The interaction of nitrogen assimilation with photosynthesis in nitrogen deficient cells of Chlorella
R J Thomas1, C R Hipkin, P J Syrett
1Department of Botany and Microbiology, University College of Swansea, Singleton Park, SA2 8PP, Swansea, U.K..
Abstract:
Nitrogen-limited chemostat cultures of Chlorella fusca var. vacuolata, when given nitrogen in the inorganic forms of nitrate, nitrite and ammonium divert photo-generated electrons, from CO2 fixation to nitrogen assimilation. Addition of nitrate or nitrite, but not ammonium, stimulates rate of oxygen evolution. All but the most severely nitrogen-deficient culture have increased dark respiration rates after addition of inorganic nitrogen. The nitrite reduction step of nitrogen assimilation is the most light-dependent reaction.
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