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Luminescent solid phase for sialic acid determination: a promising sensor for milk-adulterated samples
Aline de A Alves1, Mônica F Belian, André F Lavorante
1Departamento de Ciências Moleculares, Universidade Federal Rural de Pernambuco, 52171-900, Recife, Pernambuco, Brazil.
Luminescence : the Journal of Biological and Chemical Luminescence
|January 16, 2014
Summary
This study developed a luminescent silica material for detecting sialic acid (NANA) in adulterated milk. The novel Eu-SilTTA material demonstrates high accuracy and a low detection limit for NANA determination.
Area of Science:
- Materials Science
- Analytical Chemistry
- Spectroscopy
Background:
- Sialic acid (NANA) is a crucial biomarker in biological fluids.
- Accurate NANA determination is vital for food safety and diagnostics.
- Developing sensitive and selective detection methods remains a challenge.
Purpose of the Study:
- To synthesize and characterize a novel luminescent silica material, Eu-SilTTA.
- To investigate the potential of Eu-SilTTA for the quantitative determination of sialic acid (NANA).
- To evaluate the performance of the developed material for NANA detection in adulterated milk samples.
Main Methods:
- Synthesis of silica modified with thenoyltrifluoroacetonate (SilTTA).
- Coordination of europium (III) ion to SilTTA-functionalized silica.
- Characterization using elemental analysis and infrared spectroscopy.
- Spectroscopic analysis (emission spectra) to study Eu-SilTTA interaction with NANA.
- Batch assay for NANA determination in milk samples.
Main Results:
- Successful synthesis and characterization of Eu-SilTTA, confirming silica functionalization and coordination.
- Significant changes in emission spectra upon NANA coordination to Eu(III), indicating successful binding.
- Phenomenological intensity parameters showed increased polarizability around Eu(III) upon NANA binding.
- High correlation coefficient (0.9992) and low detection limit (0.4 mg/L) for NANA determination in adulterated milk.
- Excellent precision with a relative standard deviation (RSD%) of 0.0028.
Conclusions:
- Eu-SilTTA is a promising luminescent material for sensitive and selective sialic acid (NANA) detection.
- The developed method is effective for analyzing NANA in complex matrices like adulterated milk.
- This approach offers a viable tool for quality control and authenticity assessment in the food industry.
