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Updated: May 4, 2026
A Small Volume Bioassay to Assess Bacterial/Phytoplankton Co-culture Using WATER-Pulse-Amplitude-Modulated WATER-PAM Fluorometry
Published on: March 11, 2015
Development of a bioassay reagent usingPhotobacterium phosphoreum as a test for the detection of aquatic toxicants
1Centro de Investigaciones del Medio Ambiente (CIMA) and Centro de Investigacion y Desarrollo de Fermentaciones Industriales (CINDEFI), facultad de Ciencias Exactas, Universidad Nacional de La Plata, 47 esq. 115, 1900, La Plata, Argentina.
Abstract:
Lyophilized cells ofPhotobacterium phosphoreum, rehydrated in 2% (w/v) NaCl in 0.022M KH2PO4 at pH 7.0, were used for developing an assay to test the acute toxicity of organic and inorganic compounds. The standardized assay gave good reproducibility of results with 11 organic and four inorganic compounds. Results were compared with reported data obtained with other test organisms and are within their sensitivity ranges. Environmental screening of wastes from oil and petrochemical industries is discussed.
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