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Optimization of extracting stachyose from Stachys floridana Schuttl. ex Benth by response surface methodology
Zhong Xianfeng1, Huang Guidong1, Chen Yan2
1State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047 China ; Food Bioengineering College, University of Science and Technology of Henan, Luoyang, 471003 China.
Abstract:
Stachys floridana schuttl. E x benth, also named yinmiao, is a special and traditional Chinese vegetable that is usually used by some diabetes patients as a pharmaceutical plant for its high content of stachyose. Due to the lower cost and higher economic reward, Stachys floridana schuttl. Ex benth is a potentially new source to extract stachyose in the medicine and food industry. Here we optimized the extraction of stachyose from Stachys floridana schuttl. Ex benth by response surface methodology, the yeild can reach as high as up to 47.0% of stachyose at temperature of 60 °C, extraction time of 40 min, ethanol volume of 60% and ratio of solid-liquid at 1:10. Our primary result holds great promising for Stachys floridana schuttl. Ex bent extracting industry as well as diabetic medicine.
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Response Surface Methodology
The process of RSM involves several key steps:
Methods of Medium Optimization