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Updated: May 4, 2026

Measurement of Tissue Non-Heme Iron Content using a Bathophenanthroline-Based Colorimetric Assay
Published on: January 31, 2022
Low hemoglobin density as a measure of iron status
Sharat Damodar1, Sneha T Raghunath1, Sreedhar Murthy2
1Department of Haematology, Narayana Hrudayalaya, #258/A Bommasandra Industrial Area, Anekal Taluk, Bangalore, 560099 India.
Abstract:
Iron deficiency anemia is the commonest cause of anemia in the developing countries. Iron status is the result of the balance between the rate of erythropoiesis and the amount of iron stored in the body. Various biochemical parameters have been used to assess iron status such as iron levels, transferrin, transferrin saturation and ferritin, and all of them may be influenced by acute phase response and are also expensive tests 1-4. In our situation where patients cannot afford exhaustive tests to document iron deficiency we utilized the LHD values as a predictor of iron status based on the formula provided by Urrechaga 5.
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