Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Polyoma virus DNA replication is semi-discontinuous.

E A Hendrickson, C E Fritze, W R Folk

    Nucleic Acids Research
    |August 25, 1987
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Genome Duplication at the Beginning of Mammalian Development.

    Current topics in developmental biology·2016
    Same author

    Isolation and sequence analysis of a nuclear tRNA(met-i) gene from soybean.

    Plant molecular biology·2013
    Same author

    Why HIV positive patients on antiretroviral treatment and/or cotrimoxazole prophylaxis use traditional medicine: perceptions of health workers, traditional healers and patients: a study in two provinces of South Africa.

    African journal of traditional, complementary, and alternative medicines : AJTCAM·2013
    Same author

    Restriction fragment mass polymorphism (RFMP) analysis based on MALDI-TOF mass spectrometry for detecting antiretroviral resistance in HIV-1 infected patients.

    Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2013
    Same author

    Helix 6 of tBid is necessary but not sufficient for mitochondrial binding activity.

    Apoptosis : an international journal on programmed cell death·2003
    Same author

    Site-specific DNA binding of the Schizosaccharomyces pombe origin recognition complex is determined by the Orc4 subunit.

    Molecular and cellular biology·2001

    Polyoma virus (PyV) and simian virus 40 (SV40) DNA replication share similar mechanisms. Both viruses exhibit discontinuous DNA synthesis primarily on the retrograde template at replication forks.

    Area of Science:

    • Virology
    • Molecular Biology
    • Genetics

    Background:

    • Polyoma virus (PyV) replication was thought to be discontinuous on both replication fork arms, unlike simian virus 40 (SV40).
    • Understanding the similarities and differences in DNA replication mechanisms between closely related viruses like PyV and SV40 is crucial.

    Purpose of the Study:

    • To investigate and clarify the relationship between PyV and SV40 DNA replication mechanisms.
    • To examine the distribution and origin of RNA-primed DNA chains at replication forks in both viruses.

    Main Methods:

    • Analysis of replicating DNA purified from PyV- and SV40-infected cells.
    • Characterization of RNA-primed DNA chains, including their length, 5'-end composition, and hybridization to template strands.

    Related Experiment Videos

    Main Results:

    • Approximately one-third of PyV DNA chains and a similar fraction of SV40 DNA chains contained RNA primers (7-9 nucleotides for PyV, 6-9 for SV40).
    • Over 80% of RNA-primed DNA chains from both viruses hybridized to the retrograde template.
    • At least 95% of RNA-primed DNA chains with mapped initiation sites originated from the retrograde template.

    Conclusions:

    • PyV and SV40 DNA replication forks exhibit essentially the same mechanism.
    • DNA synthesis in both viruses is predominantly, if not exclusively, discontinuous on the retrograde template.