Crambe tataria: the glucosinolate/myrosinase system in vivo and in vitro

The Crambe tataria glucosinolate/myrosinase system in seeds and leaves of in vivo and in vitro regenerated plantlets, and two callus cell lines was investigated. It was demonstrated that in all the extracts glucosinolates were present and the myrosinase system was operative. There appears to be no discrimination between the glucosinolates used as substrates, but the hydrolysis rates were different regardless of the nature of the side chain. This is one of the first studies demonstrating that undifferentiated cells are able to synthetize glucosinolates and have an operating myrosinase system.