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Updated: May 4, 2026

Evaluation of Photosynthetic Behaviors by Simultaneous Measurements of Leaf Reflectance and Chlorophyll Fluorescence Analyses
Published on: August 9, 2019
Rapid, nondestructive measurement of chlorophyll content in leaves with nonuniform chlorophyll distribution
L A Spomer1, M A Smith, J S Sawwan
1Department of Horticulture, University of Illinois, 61801, Urbana, IL, USA.
Abstract:
A practical microcomputerized video image analysis method is described for quantifying leaf chlorophyll content without extraction. Chlorophyll concentration is estimated from densimetric measurements of whole, intact leaves. Direct comparison with conventional extraction measurements on Epipremnum aureum, a variegated species, verified the image analysis technique's accuracy. The inherent advantages with regard to the nondestructive and convenient nature of the measurement, and suitability for leaves with irregular chlorophyll distribution, are discussed.

