Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Studies on Streptococcus faecalis enterotoxin.

M S Kalra, G Kaur, A Singh

    Acta Microbiologica Polonica
    |January 1, 1987
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Evaluation of the modulatory influence of food additive-garam masala on hepatic detoxication system.

    Indian journal of experimental biology·1992
    Same author

    Cutaneous toxicity of sodium lauryl sulphate, nickel, and their combination in guinea pigs: biochemical and histopathological observations.

    Bulletin of environmental contamination and toxicology·1992
    Same author

    RNase X2, a pistil-specific ribonuclease from Petunia inflata, shares sequence similarity with solanaceous S proteins.

    Plant molecular biology·1992
    Same author

    Dermal toxicity of paraphenylenediamine.

    Biomedical and environmental sciences : BES·1992
    Same author

    Modulation of bilayer structures derived from diacetylenic phosphocholines containing oxygen linker beta to diacetylene.

    Chemistry and physics of lipids·1992
    Same author

    The familial occurrence of Parkinson's disease. Lack of evidence for maternal inheritance.

    Archives of neurology·1992
    Same journal

    Changes in murein composition during the cell cycle of Caulobacter crescentus.

    Acta microbiologica Polonica·2011
    Same journal

    The murein of Thiobacillus neapolitanus.

    Acta microbiologica Polonica·2011
    Same journal

    Characterization of the cell wall murein of Thiobacillus versutus.

    Acta microbiologica Polonica·2011
    Same journal

    Mutants of Caulobacter crescentus resistant to beta-lactam antibiotics.

    Acta microbiologica Polonica·2011
    Same journal

    Characterization of a Helical Morphological Mutant of Caulobacter crescentus.

    Acta microbiologica Polonica·2011
    Same journal

    Intracellular localization of the ATP-dependent deoxyribonuclease of Caulobacter crescentus.

    Acta microbiologica Polonica·2009
    See all related articles

    This study investigated Streptococcus faecalis strains causing food poisoning. Researchers identified enterotoxin properties and antibiotic sensitivities, finding some mutants lost toxin production.

    Area of Science:

    • Microbiology
    • Food Safety
    • Bacterial Pathogenesis

    Background:

    • Streptococcus faecalis, now often classified as Enterococcus faecalis, is a known cause of foodborne illness.
    • Understanding the characteristics of enterotoxin-producing strains is crucial for food safety.
    • Previous research has indicated the potential for S. faecalis to cause food poisoning.

    Purpose of the Study:

    • To characterize enterotoxin production in S. faecalis strains.
    • To determine the enzymatic activity and optimal conditions for the enterotoxin.
    • To assess the antibiotic susceptibility of S. faecalis strains and identify mutants with altered toxin production.

    Main Methods:

    • Six S. faecalis strains were evaluated using Sherman's criteria.
    • Enterotoxin was purified using Sephadex G-200 chromatography.

    Related Experiment Videos

  • Enzyme activity was assessed using trypsin and papain.
  • Antibiotic sensitivity testing was performed on all strains.
  • Mutagenesis was induced using acridine orange and ethidium bromide.

    • Main Results:

    • All tested S. faecalis strains met Sherman's criteria, being non-hemolytic, DNase-positive, and enterotoxin-positive (Ent+).
    • The purified enterotoxin showed maximum activity at 37°C and pH 7.0.
    • Enterotoxin treated with proteases (trypsin, papain) exhibited reduced fluid accumulation response.
    • Antibiotic sensitivity varied among strains.
    • Mutagenesis yielded 47 mutants; 4 were toxin-negative, with 3 also being DNase-negative and 1 showing partial DNase activity.

    Conclusions:

    • The study confirms the enterotoxigenic potential of S. faecalis strains.
    • Proteolytic enzymes significantly impact enterotoxin activity.
    • Mutagenesis offers a method to investigate the genetic basis of toxin production, with some mutants losing both enterotoxin and DNase activity.