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The hybridisation of [(3)H]poly(uridylic) acid to DNA from Phaseolus aureus Roxb

¹Department of Physiology and Environmental Studies, University of Nottingham, School of Agriculture, Sutton Bonington, LE12 5RD, Loughborough, U.K..

|January 17, 2014

Abstract:

[5-(3)H]poly(uridylic) acid hybridises to nuclear DNA, suggesting the DNA contains poly (deoxyadenylic) acid sequences. These regions do not appear to be clustered in the DNA. Although the amount of DNA involved is small it is sufficient for thousands of sequences. Thermal dissociation of the hybrids shows that a relatively small number of hydrogen bonds is involved in each and it is concluded that these sequences are relatively short. In view of the fact that much longer poly(adenylic) acid sequences are found in plant RNA it is concluded that these are added after transcription.

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