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A gibberellin bioassay based on betacyanin production in Amaranthus caudatus seedlings
L T Kinsman1, N J Pinfield, A K Stobart
1Plant Cell Metabolism Laboratory, Department of Botany, The University, BS8 1UG, Bristol, UK.
Abstract:
Amaranthus caudatus L. seedlings produce less amaranthin in the presence of gibberellic acid A3 (GA3). This phenomenon has been tested as a bioassay for gibberellic acid and has been compared to the bioassay based on lettuce hypocotyl extension. The inhibition of pigment synthesis is much more sensitive to GA3 at concentrations from 1 to 0.01 μg/ml. Of the gibberellins tested GA1, GA3, GA4, and GA7 are the most active in the described bioassay.
