Related Experiment Video
Updated: May 4, 2026

Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics
Published on: October 19, 2018
Relative thermostability of the chloroplast envelope
1Botanisches Institute der Universität Düsseldorf, D-4000, Düsseldorf 1, Germany.
Abstract:
Intact isolated chloroplasts from leaves of Spinacia oleracea L. were subjected to heat treatment. After heating, the integrity of the chloroplast envelopes and the activities of various light-dependent chloroplast reactions were tested. The integrity of the chloroplast envelopes, as judged from rates of ferricyanide reduction, enzyme compartmentation and visual appearance of the chloroplasts in the light microscope with phase optics, was affected much less by heat stress than the photochemical reactions of thylakoids. This indicates a comparatively high thermostability of the chloroplast envelope membranes. It is also evidence of a differential thermostability of different biomembranes. Photophosphorylation was highly susceptible to thermal stress. Heat treatment that partly inactivated phosphorylation stimulated light-dependent quenching of 9-aminoacridine fluorescence, which served as an indicator of proton transfer from stroma to thylakoids in intact chloroplasts. Drastic changes in the characteristics of chlorophyll a fluorescence emission caused by heating were probably due to structural alterations of the thylakoid system.
More Related Videos
12:25Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays
Published on: September 28, 2018
08:04A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry
Published on: March 13, 2014
Related Concept Videos
Protein Transport to the Inner Chloroplast Membrane
Responses to Heat and Cold Stress
Diversity of Archaea IV
Protein Transport to the Stroma
Protein complexes called the translocon of the outer chloroplast membrane or TOC complex, and the translocon of the inner chloroplast membrane or TIC complex mediate the...
Protein Transport to the Outer Chloroplast Membrane
Two models describe the mechanism of precursor recognition and entry across the outer membrane through the TOC complex. Model 1 suggests the newly synthesized precursor binds to the TOC receptor 159 and forms a complex.
Membrane Fluidity