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Author Spotlight: Optimizing Apoplast Protein Extraction for Efficient Recovery of Recombinant Proteins from Plant Cells
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An improvement of auxin extraction procedure and its application to cultured plant cells
S Atsumi1, S Kuraishi, T Hayashi
1Department of Biology, College of General Education, University of Tokyo, Komaba, Meguro, 153, Tokyo, Japan.
Planta
|January 17, 2014
Abstract:
The senescent cultured tobacco (Nicotiana tabacum L.) cells, XD6S, when extracted with customary procedure, contained much auxin. The use of new extraction procedure using dichloromethane which does not extract much indolepyruvic acid revealed that the cultured tobacco cells do not contain measurable auxin at any stage of culture.
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