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Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis
Published on: July 16, 2019
[Mutations in Oenothera hookeri after treatment of the pollen with radio waves]
1Institut für Entwicklungsphysiologie, Universität zu Köln, BRD.
Abstract:
Pollen of Oenothera hookeri was exposed to radio waves (λ = 1,5 m; 1,4 and 1,8 mV/m) for 4 and 12 hrs. This pollen was crossed to normal emasculated flowers. The M 1-generation and the M 2- and M 3-generations were investigated. The criteria for a mutagenic action of the treatment were: haploid plants in M 1; partial pollen lethality in M 1, some due to chromosomal abnormalities; genetic heterogeneity of M 1 plants for lethal genes and for genes for reduced vitality as determined by the frequency of seeds with lethal embryos in M 2; segregation of morphological abnormalities in M 2. All experiments demonstrated the mutagenic effect of the radio wave treatment.
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