Aptamer-based portable biosensor for platelet-derived growth factor-BB (PDGF-BB) with personal glucose meter readout

Area of Science:

• Biotechnology
• Biosensor Development
• Analytical Chemistry

Background:

• Platelet-derived growth factor-BB (PDGF-BB) is a significant biomarker.
• Accurate and sensitive detection methods for PDGF-BB are crucial for diagnostics.
• Existing methods may lack portability or sensitivity for certain applications.

Purpose of the Study:

• To develop a novel portable biosensor for sensitive and selective PDGF-BB detection.
• To utilize a personal glucose meter (PGM) as a readout for enhanced accessibility.
• To validate the biosensor's performance in biological samples like saliva.

Main Methods:

• A biosensor was designed using aptamer-protein recognition.
• Streptavidin magnespheres paramagnetic particles (SA-PMPs) functionalized with PDGF-BB aptamers were used.
• Invertase-functionalized secondary aptamers formed a complex, generating a glucose signal detected by a PGM.

Main Results:

• The biosensor demonstrated a quantitative relationship between PGM signal and PDGF-BB concentration (1.0 × 10(-14) M to 3.16 × 10(-12) M).
• Achieved a highly sensitive detection limit of 2.9 fM.
• Successfully applied the biosensor for PDGF-BB assay in human saliva samples.

Conclusions:

• A novel, portable aptamer-based biosensor for PDGF-BB detection was successfully developed.
• The PGM readout offers a cost-effective and accessible detection platform.
• The biosensor shows promise for clinical diagnostics and monitoring of PDGF-BB in saliva.