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Updated: May 3, 2026

Identification of the Source of Secreted Proteins in the Kidney by Brefeldin A Injection
Published on: November 10, 2021
Seeing is believing: dynamic changes in renal epithelial autophagy during injury and repair
Fangming Lin1, Zhao V Wang2, Joseph A Hill3
1Department of Pediatrics; Columbia University College of Physicians and Surgeons; and.
Abstract:
Ischemic injury to the kidneys is a prevalent clinical problem, contributing importantly to chronic kidney disease. Yet, underlying molecular mechanisms are elusive. To address the possible role of autophagy, we engineered a novel strain of mice harboring a ubiquitously expressed CAG-RFP-EGFP-LC3 transgene. Using this tool, we examined the post-ischemic kidney and detailed the dynamics of renal tubular epithelial autophagy. In addition, we defined the role of MTOR in the resolution of autophagy during epithelial survival and kidney repair.
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