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Related Concept Videos

Controlled-Current Coulometry: Overview01:27

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Controlled current coulometry, also known as amperostatic coulometry, is a technique used in electrochemical analysis to measure the quantity of a substance through the controlled passage of current. It involves the application of a constant current to an electrochemical cell containing the analyte of interest. As the current flows through the cell, the analyte undergoes a redox reaction at the electrode surface, resulting in a charge transfer. By monitoring the time required for a certain...
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Controlled-Current Coulometry: Coulometric Titration01:18

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Coulometric titrations are a form of titrimetric analysis where the reagent is generated electrically, and its amount is evaluated based on current and generating time. The electron serves as the standard reagent. The procedure is similar to conventional titrations, such as endpoint detection.
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Related Experiment Video

Updated: May 3, 2026

Quantification of Endogenous Auxin and Cytokinin During Internode Culture of Ipecac
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A quantitative analysis of cytokinin using single-ion-current-monitoring.

A G Thompson1, R Horgan, J K Heald

  • 1Department of Botany and Microbiology, University College of Wales, SY23 3DA, Aberystwyth, UK.

Planta
|January 18, 2014
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Summary

Researchers measured cytokinin levels in poplar leaves using single-ion current monitoring. This study quantifies 6-(o-hydroxybenzylamino)-9-β-D-ribofuranosylpurine (o-OH BAP riboside) in plant tissues.

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Area of Science:

  • Plant Physiology
  • Biochemistry
  • Analytical Chemistry

Background:

  • Cytokinins are essential plant hormones regulating growth and development.
  • Accurate quantification of cytokinin levels is crucial for understanding plant responses.
  • Poplar trees (Populus x robusta Schneid) are important model organisms in plant science.

Purpose of the Study:

  • To quantify the levels of 6-(o-hydroxybenzylamino)-9-β-D-ribofuranosylpurine (o-OH BAP riboside) in attached poplar leaves.
  • To establish a reliable method for cytokinin measurement in plant tissues.

Main Methods:

  • Utilized single-ion current monitoring (SICM) for sensitive detection.
  • Employed 6-(p-hydroxybenzylamino)-9-β-D-ribofuranosylpurine (p-OH BAP riboside) as an internal standard for quantitative recovery measurements.

Main Results:

  • Successfully measured the endogenous levels of o-OH BAP riboside in poplar leaves.
  • Demonstrated the efficacy of SICM coupled with an internal standard for accurate cytokinin quantification.

Conclusions:

  • Single-ion current monitoring is a viable technique for precise cytokinin analysis in plants.
  • This method provides a foundation for further studies on cytokinin roles in poplar physiology.