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Separation and characterisation of glycoproteins from normal, pregnancy, and acute inflammatory sera
A M Cox1, R Turner, E H Cooper
1Unit for Cancer Research, University of Leeds, U.K.
Journal of Chromatography
|June 26, 1987
Abstract:
A system of multiple chromatography combining anion-exchange, gel filtration, and affinity chromatography has been devised to separate several acute phase proteins from serum, including alpha 1 acid glycoprotein, transferrin, alpha 1-antichymotrypsin, alpha 1-antitrypsin and haemopexin. Only 5-15 ml of blood is required to provide milligram quantities of the purified proteins for further biochemical analysis. The system has been applied in a study of pregnancy and some diseases in order to investigate the changes in the serum protein profile and in individual proteins.