Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cytoplasm01:24

Cytoplasm

6.8K
The cytoplasm consists of organelles and a framework of protein scaffolds called the cytoskeleton suspended in an aqueous solution, the cytosol. The cytosol is a rich broth of water, ions, salts, and various organic molecules.
Protein Folding and Misfolding
The cytoplasm is the location for several cellular processes, including protein synthesis and folding. The aqueous nature of the cytosol promotes protein folding such that the hydrophobic amino acid side chains are buried in the protein...
6.8K
Cytoplasm01:16

Cytoplasm

66.3K
The cytoplasm consists of organelles and a framework of protein scaffolds called the cytoskeleton suspended in an aqueous solution, the cytosol. The cytosol is a rich broth of water, ions, salts, and various organic molecules.
Protein Folding and Misfolding
The cytoplasm is the location for several cellular processes, including protein synthesis and folding. The aqueous nature of the cytosol promotes protein folding such that the hydrophobic amino acid side chains are buried in the protein...
66.3K
Photosystems01:32

Photosystems

7.0K
Photosystems are multiprotein complexes that form the functional units of photosynthesis in plants, algae, and cyanobacteria. They are found embedded in the membrane of tiny sac-like structures called thylakoids placed inside the chloroplast.
Functioning of Photosystems
Photosystems contain many pigment molecules, such as chlorophylls and carotenoids, arranged in a particular organization across two domains — the antenna complex and the reaction center. The main aim of the pigment...
7.0K
What are Lipids?01:31

What are Lipids?

8.5K
Lipids function as structural components of cellular membranes, in addition to acting as energy reservoirs and signaling molecules. They are thus crucial to all living organisms.  The three biologically important classes of lipids are triglycerides, phospholipids, and steroids.
Non-Polar and Hydrophobic Characteristics of Lipids
Lipids are a structurally and functionally diverse group of hydrocarbons—compounds consisting of carbon and hydrogen atoms. The carbon-carbon and...
8.5K
What are Lipids?01:38

What are Lipids?

182.3K
Overview
182.3K
Asymmetric Lipid Bilayer01:35

Asymmetric Lipid Bilayer

8.0K
Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
8.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exciton interactions in phycoerythrin.

Photosynthesis research·2014
Same author

Regulation of the plant-type 5'-adenylyl sulfate reductase by oxidative stress.

Biochemistry·2001
Same author

Salt and oxidative stress: similar and specific responses and their relation to salt tolerance in citrus.

Planta·1998
Same author

Molecular cloning and characterization of the genes encoding the L1 and L2 components of hemolysin BL from Bacillus cereus.

Journal of bacteriology·1997
Same author

Kinetic and spectral properties of pea cytosolic ascorbate peroxidase.

FEBS letters·1996
Same author

Molecular cloning and characterization of a cDNA encoding pea monodehydroascorbate reductase.

The Journal of biological chemistry·1994

Related Experiment Video

Updated: May 3, 2026

Isolation and Characterization of Intact Phycobilisome in Cyanobacteria
06:26

Isolation and Characterization of Intact Phycobilisome in Cyanobacteria

Published on: November 10, 2021

4.0K

Phycobilisome structure and function.

B A Zilinskas1, L S Greenwald

  • 1Department of Biochemistry and Microbiology, Cook College, Rutgers University, 08903, New Brunswick, NJ, USA.

Photosynthesis Research
|January 18, 2014
PubMed
Summary
This summary is machine-generated.

Phycobilisomes, light-harvesting protein aggregates in algae, efficiently transfer energy via a defined structure. Recent advances enable in vitro assembly and genetic analysis, aiding understanding of their function.

More Related Videos

Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis
08:07

Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis

Published on: September 11, 2018

15.0K
Purification of Active Photosystem I-Light Harvesting Complex I from Plant Tissues
07:10

Purification of Active Photosystem I-Light Harvesting Complex I from Plant Tissues

Published on: February 3, 2023

1.5K

Related Experiment Videos

Last Updated: May 3, 2026

Isolation and Characterization of Intact Phycobilisome in Cyanobacteria
06:26

Isolation and Characterization of Intact Phycobilisome in Cyanobacteria

Published on: November 10, 2021

4.0K
Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis
08:07

Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis

Published on: September 11, 2018

15.0K
Purification of Active Photosystem I-Light Harvesting Complex I from Plant Tissues
07:10

Purification of Active Photosystem I-Light Harvesting Complex I from Plant Tissues

Published on: February 3, 2023

1.5K

Area of Science:

  • Photosynthesis and light harvesting in algae
  • Molecular and structural biology of protein complexes

Background:

  • Phycobilisomes are essential light-harvesting antenna complexes in cyanobacteria and red algae.
  • They are composed of phycobiliproteins and linker proteins, attached to thylakoid membranes.
  • Efficient energy transfer occurs from phycoerythrin to phycocyanin, then to allophycocyanin.

Purpose of the Study:

  • To elucidate the ultrastructure and energy transfer pathways within phycobilisomes.
  • To understand the assembly mechanisms and interactions with thylakoid membranes.
  • To explore the potential for in vitro assembly and genetic manipulation of phycobilisomes.

Main Methods:

  • Combined ultrastructural, biochemical, and biophysical approaches.
  • Time-resolved spectroscopic measurements to confirm energy transfer pathways.
  • In vitro assembly of functional phycobilisomes from separated components.
  • Molecular genetics for identifying genes encoding phycobilisome components.

Main Results:

  • A detailed model of phycobilisome structure, featuring an allophycocyanin core and peripheral rods of phycocyanin and phycoerythrin.
  • Confirmation of near 100% excitation energy transfer efficiency through the phycobilisome antenna.
  • Successful in vitro assembly of functional phycobilisomes and reassociation with thylakoids.
  • Identification of genes encoding key phycobilisome components.

Conclusions:

  • The ordered structure of phycobilisomes is critical for highly efficient light energy transfer.
  • Advances in molecular genetics and in vitro assembly provide powerful tools for studying phycobilisome biosynthesis and function.
  • Further research is needed to fully understand phycobilisome-thylakoid interactions and energy distribution regulation.