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Updated: May 3, 2026

Purification of Active Photosystem I-Light Harvesting Complex I from Plant Tissues
Published on: February 3, 2023
Selective photobleaching of PSI-related chlorophylls in heat-stressed pea chloroplasts
W P Williams1, A Sen, D C Fork
1Department of Plant Biology, Carnegie Institution of Washington, 290 Panama Street, 94305, Stanford, CA, USA.
Abstract:
Measurements of electron transport activity point to the occurrence of major changes in the organisation of the photosynthetic apparatus of heat-stressed chloroplasts. One of the consequences of these changes is shown to be a greatly increased susceptibility of chlorophyll to photobleaching. Despite the fact that the threshold temperature for this photobleaching coincides closely with that for the inhibition of PSII activity, the bleached components were found to be specifically associated with PSI. This increased susceptibility of PSI pigments to photobleaching is shown to be a direct consequence of an interruption of the flow of reductants from PSII to PSI that would normally protect PSI from photooxidation.
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