Modification of oxygen evolving center by Tris-washing
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Updated: May 3, 2026

Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase
Published on: November 8, 2019
1Institute of Biological Sciences, Tsukuba University, Sakura-mura, 305, Ibaraki, Japan.
Abstract:
Tris-washing inhibits the O2-evolving center of chloroplasts and their particles specifically and reversibly, and it was applied to many investigations on O2-evolving center and PS II reaction center. In this review are introduced the various photosynthetic investigations in which Tris-washing was applied and are also discussed briefly on the site and the mechanism of Tris-inactivation, properties of P680 and Z, characteristic change in fluorescence and delayed light emission, and reactivation of O2-evolving center by DCPIP.H2-treatment and photo-reactivation of Tris-washed chloroplasts and their particles.
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