Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Strategies for epitope analysis using peptide synthesis.

H M Geysen1, S J Rodda, T J Mason

  • 1Department of Molecular Immunology, Commonwealth Serum Laboratories, Parkville, Victoria, Australia.

Journal of Immunological Methods
|September 24, 1987
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Australia's temperate highland peat swamps face a death by a thousand longwall cuts.

Journal of environmental management·2025
Same author

Conserving mire ecosystems: linking flammability and moisture content of peaty substrates.

The Science of the total environment·2025
Same author

Persistent effects of underground longwall coal mining on freshwater wetland hydrology.

The Science of the total environment·2021
Same author

Ultrasonic, hydrodynamic and microwave biodiesel synthesis - A comparative study for continuous process.

Ultrasonics sonochemistry·2019
Same author

A reactor designed for the ultrasonic stimulation of enzymatic esterification.

Ultrasonics sonochemistry·2019
Same author

Detecting state changes for ecosystem conservation with long-term monitoring of species composition.

Ecological applications : a publication of the Ecological Society of America·2017
Same journal

Optimized intracellular flow cytometry panel enables CD4 and CD8 T cell cytokine profiling in Syrian hamsters.

Journal of immunological methods·2026
Same journal

Isosulfan blue sentinel lymph node biopsy enables reliable lymph node harvest and multicolor flow cytometry in mice.

Journal of immunological methods·2026
Same journal

Type-specific antibody detection of herpes simplex virus types 1&2 (HSV-1&2) in fingerstick blood at point-of-care sites by a rapid and sensitive lateral flow immunochromatographic assay.

Journal of immunological methods·2026
Same journal

Development of chimeric DGP-IgG antibodies as quality control for celiac disease diagnosis.

Journal of immunological methods·2026
Same journal

Stepwise single-cell-resolved deep immunophenotyping pipeline to characterise immune heterogeneity and functionality in health and disease.

Journal of immunological methods·2026
Same journal

Performance evaluation of Meso Scale Discovery (MSD) quantitative serological assays for detection of binding (IgG, IgA, IgM) and ACE2 inhibitory antibody levels for SARS-CoV-2.

Journal of immunological methods·2026
See all related articles

A new method for synthesizing and screening peptides enables detailed analysis of antigenic determinants. This approach allows for precise mapping of epitopes and identification of key amino acid residues for any monoclonal antibody.

Area of Science:

  • Immunology
  • Biochemistry
  • Peptide Chemistry

Background:

  • Understanding antigenic determinants is crucial for vaccine development and diagnostics.
  • Previous methods for mapping epitopes were often laborious and limited in scope.
  • The need for high-throughput techniques to analyze protein-antigen interactions is significant.

Purpose of the Study:

  • To describe a novel approach for the synthesis and enzyme-linked immunosorbent assay (ELISA) screening of large peptide libraries.
  • To enable the detailed investigation of antigenic determinant sites and specificity.
  • To develop strategies for rapid epitope mapping and characterization.

Main Methods:

  • High-throughput synthesis of peptide libraries.
  • Enzyme-linked immunosorbent assay (ELISA) for screening peptide-antigen interactions.

Related Experiment Videos

  • Development of specific strategies for locating continuous antigenic peptides.
  • Identification of critical amino acid residues within antigenic peptides.
  • Determination of amino acid residues involved in monoclonal antibody binding.

    • Main Results:

    • Successful application of the method for locating all continuous antigenic peptides of a protein antigen.
    • Identification of non-replaceable amino acid residues essential for antigenicity.
    • Development of a procedure to determine the epitope recognized by any monoclonal antibody.
    • Demonstration of the method's utility in tackling previously intractable questions in immunochemistry.

    Conclusions:

    • The described method provides a powerful tool for dissecting antigen-antibody interactions.
    • This approach facilitates the rapid mapping of antigenic properties of poorly understood antigens.
    • The findings have significant implications for epitope-based vaccine design and diagnostic development.