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3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening
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Shear reversible cell/microsphere aggregate as an injectable for tissue regeneration.

Eunkyeong Woo1, Honghyun Park, Kuen Yong Lee

  • 1Department of Bioengineering, Hanyang University, Seoul, 133-791, Republic of Korea.

Macromolecular Bioscience
|January 18, 2014
PubMed
Summary

Injectable alginate microspheres, modified with RGD peptides, form shear-reversible cell aggregates. These aggregates enable minimally invasive delivery of chondrocytes for effective in vivo cartilage tissue regeneration.

Keywords:
aggregatealginatecartilage regenerationmicrospheretissue engineering

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Injectable delivery systems are crucial for minimally invasive cell delivery in tissue engineering.
  • Developing effective methods for cell delivery and tissue regeneration remains a significant challenge.

Purpose of the Study:

  • To investigate the potential of cell-sized microspheres to form shear-reversible aggregates with cells.
  • To evaluate the utility of these cell-microsphere aggregates for injectable delivery and in vivo tissue engineering.

Main Methods:

  • Alginate microspheres were fabricated using an emulsion method.
  • Microspheres were modified with arginine-glycine-aspartic acid (RGD) peptide sequences.
  • Aggregation behavior of RGD-modified microspheres with chondrocytes was assessed under shear conditions.

Main Results:

  • RGD-modified alginate microspheres successfully formed shear-reversible aggregates with chondrocytes.
  • These cell/microsphere aggregates demonstrated suitability for syringe-based delivery into an animal model.
  • The injectable aggregates promoted effective in vivo cartilage tissue regeneration.

Conclusions:

  • Cell-sized, RGD-modified alginate microspheres can form injectable, shear-reversible cell aggregates.
  • This approach offers a promising strategy for minimally invasive delivery of chondrocytes for cartilage repair.
  • The study highlights the potential of engineered microspheres in advancing tissue engineering applications.