Structure elucidation of DNA-protein crosslinks by using reductive desulfurization and liquid chromatography-tandem
Susith Wickramaratne1, Natalia Y Tretyakova
1University of Minnesota Masonic Cancer Center and the Department of Chemistry, 2231 6th Street SE, Room 2-220 CCRB, Minneapolis, MN 55455 (USA).
Abstract:
Easier with ethyl: Guengerich and co-workers have developed a powerful new approach to the structure elucidation of hydrolytically stable AGT-DNA crosslinks by reductive desulfurization of the thioether linkage between AGT and DNA to convert cysteine DPCs to the corresponding ethyl-DNA adducts, which can be readily characterized by LC-MSn.
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