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Measurement of thyroxine and cortisol in canine and feline blood samples using two immunoassay analysers
1Small Animal Hospital, School of Veterinary Sciences, University of Bristol, Langford, Bristol, BS40 5DU.
Objectives:
The AIA-360 (Tosoh Corporation) is an automated immunoassay analyser. The aims of this study were to estimate the precision of thyroxine and cortisol AIA-360 immunoassays in canine and feline samples and to compare the results produced with those obtained by a chemiluminescence analyser (Immulite® 1000, Siemens).
Methods:
Blood samples from 240 clinical cases (60 dogs and 60 cats for both thyroxine and cortisol) were analysed using both instruments.
Results:
Deming regression calculations showed excellent correlation (thyroxine, canine rs = 0 · 94, feline rs = 0 · 97; cortisol, canine rs = 0 · 97, feline rs = 0 · 97). Agreement between the two instruments was examined by Bland-Altman difference plots, which identified wide confidence intervals and outliers for thyroxine (canine n = 6, feline n = 4) and cortisol (canine n = 3, feline n = 4) results. Inter/intra-run precision of the AIA-360 was excellent for both cortisol and thyroxine (coefficients of variation <7%).
Clinical Significance:
The instrument showed excellent correlation for cortisol and thyroxine in canine and feline samples demonstrating that the AIA-360 can be used in clinical practice. The agreement studies suggest that the results from the AIA-360 cannot be used interchangeably with those generated by the Immulite 1000 and should be interpreted using reference intervals that have been established specific to the AIA-360.
